3ULP
Plasmodium falciparum SSB complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.017, 82.794, 87.556 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.22084 |
| Rwork | 0.218 |
| R-free | 0.27557 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eqq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.529 |
| Number of reflections | 48456 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 99.4 | 97.3 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Bis-Tris Propane, pH 8.5, 20 % PEG 3350, 0.2 M sodium bromide, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
