3ULL
HUMAN MITOCHONDRIAL SINGLE-STRANDED DNA BINDING PROTEIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | RIGAKU |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 51.830, 51.830, 184.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 * - 2.400 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23700 |
| Structure solution method | MIR |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.210 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.055 | 0.064 |
| Number of reflections | 9017 | |
| <I/σ(I)> | 10 | 2.5 |
| Completeness [%] | 92.0 | 80.8 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | 4 * | Hilgenfeld, R., (1984) FEBS Letts., 170, 143. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | 0.5-0.7 (M) | ||
| 3 | 1 | drop | phosphate | 20 (mM) | |
| 4 | 1 | drop | 3 (mM) | pH7.0 | |
| 5 | 1 | reservoir | phosphate | 20-60 (mM) |
