3ULJ
Crystal structure of apo Lin28B cold shock domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-29 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.98141 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.620, 52.620, 137.336 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.300 - 1.060 |
| R-factor | 0.1206 |
| Rwork | 0.120 |
| R-free | 0.13910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 312Z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.573 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 1.087 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmerge | 0.056 | 0.610 |
| Number of reflections | 100170 | |
| <I/σ(I)> | 17.8 | 2.8 |
| Completeness [%] | 99.5 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 2.5 M sodium acetate, 0.1 M HEPES , pH 7.0, EVAPORATION |
