3ULH

Crystal structure of a RNA binding domain of THO complex subunit 4 protein (THOC4) from Homo sapiens at 2.54 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537,0.9796,0.9794
Spacegroup name	P 41 3 2
Unit cell lengths	101.152, 101.152, 101.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 - 2.540
R-factor	0.1819
Rwork	0.181
R-free	0.19540
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.958
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.200	29.200	2.610
High resolution limit [Å]	2.540	11.360	2.540
Rmerge		0.011	0.011
Total number of observations		687	4595
Number of reflections	6271
<I/σ(I)>	16.6	30.9	2.2
Completeness [%]	99.9	94.2	100
Redundancy	9.8	7.4	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25.0% Glycerol, 0.6M di-sodium hydrogen phosphate, 0.6M potassium dihydrogen phosphate, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K