3ULD
High resolution structure of DNA/RNA hybrid in complex with RNase H catalytic domain D132N mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9765 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.301, 37.672, 62.378 |
Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.19589 |
Rwork | 0.194 |
R-free | 0.23463 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3twh |
RMSD bond length | 0.012 |
RMSD bond angle | 1.560 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.940 | 1.639 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.080 | 0.080 |
Number of reflections | 23657 | |
<I/σ(I)> | 16.9 | 16.9 |
Completeness [%] | 94.1 | 94.1 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.04 M Magnesium chloride, 0.05 M Sodium cacodylate, 5% MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |