3UL4
Crystal structure of Coh-OlpA(Cthe_3080)-Doc918(Cthe_0918) complex: A novel type I Cohesin-Dockerin complex from Clostridium thermocellum ATTC 27405
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-24 |
Detector | ADSC QUANTUM 4r |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 130.050, 130.050, 70.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.790 - 1.950 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.314 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | BALBES |
Refinement software | PHENIX (1.7.2_865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.959 | 2.050 |
High resolution limit [Å] | 1.946 | 1.950 |
Rmerge | 0.420 | |
Number of reflections | 22341 | |
<I/σ(I)> | 23.3 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.4 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 293 | 0.2 M lithium sulfate, 10% w/v PEG 8000+, 10% w/v PEG 1000, pH 7.5, vapor diffusion, hanging drop, temperature 293K |