3UL3
Structural insights into thioredoxin-2: a component of malaria parasite protein secretion machinery
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.046, 87.046, 90.904 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.256 - 2.905 |
| R-factor | 0.2612 |
| Rwork | 0.257 |
| R-free | 0.29760 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.688 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.250 | 2.900 |
| Rmerge | 0.040 | 0.018 | 0.366 |
| Number of reflections | 8947 | ||
| <I/σ(I)> | 15.1 | ||
| Completeness [%] | 98.2 | 94.3 | 99.1 |
| Redundancy | 3.7 | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.125M Bis-tris pH 5.5, 2.2M ammonium sulfate, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
