3UL2
Galactose-specific lectin from Dolichos lablab in P6522 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.496, 90.496, 514.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.27459 |
| Rwork | 0.273 |
| R-free | 0.29624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.045 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.101 | 0.448 |
| Number of reflections | 44836 | |
| <I/σ(I)> | 27.1 | 2.7 |
| Completeness [%] | 93.4 | 95.7 |
| Redundancy | 9.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 30% PEG 4K, 100mM Tris, 200mM Lithium sulphate , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
