3UKY

Mouse importin alpha: yeast CBP80 cNLS complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953693
Spacegroup name	P 21 21 21
Unit cell lengths	78.195, 89.747, 98.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.180 - 2.350
R-factor	0.1894
Rwork	0.188
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pjn
RMSD bond length	0.002
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.570
High resolution limit [Å]	2.350
Number of reflections	29432
Completeness [%]	68.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.8M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K