3UKW
Mouse importin alpha: Bimax1 peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.953693 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.561, 89.493, 99.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.151 - 2.100 |
R-factor | 0.1777 |
Rwork | 0.176 |
R-free | 0.19960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pjn |
RMSD bond length | 0.004 |
RMSD bond angle | 0.789 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.600 |
High resolution limit [Å] | 2.100 |
Number of reflections | 41865 |
Completeness [%] | 76.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.8M sodium citrate, 10mM DTT, 0.1M BisTris, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |