3UKW

Mouse importin alpha: Bimax1 peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953693
Spacegroup name	P 21 21 21
Unit cell lengths	78.561, 89.493, 99.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.151 - 2.100
R-factor	0.1777
Rwork	0.176
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pjn
RMSD bond length	0.004
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.600
High resolution limit [Å]	2.100
Number of reflections	41865
Completeness [%]	76.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.8M sodium citrate, 10mM DTT, 0.1M BisTris, 10% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K