3UKV
Structure of the C-linker/CNBHD of zELK channels in P 1 21 1 space group, crystallized in the presence of cAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.330, 106.420, 77.520 |
| Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
| Resolution | 48.759 - 2.700 |
| R-factor | 0.2183 |
| Rwork | 0.215 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF THE CORRESPONDING SELENOMETHIONINE DERIVATIVE CRYSTAL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.253 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.066 | 0.453 |
| Number of reflections | 24491 | |
| <I/σ(I)> | 8.1 | 1.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 5 mM cAMP, 180 mM ammonium acetate, 22.5% (w/v) PEG 3350, 90 mM TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
