3UKV
Structure of the C-linker/CNBHD of zELK channels in P 1 21 1 space group, crystallized in the presence of cAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-07-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.330, 106.420, 77.520 |
Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
Resolution | 48.759 - 2.700 |
R-factor | 0.2183 |
Rwork | 0.215 |
R-free | 0.27680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STRUCTURE OF THE CORRESPONDING SELENOMETHIONINE DERIVATIVE CRYSTAL |
RMSD bond length | 0.009 |
RMSD bond angle | 1.253 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.760 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.066 | 0.453 |
Number of reflections | 24491 | |
<I/σ(I)> | 8.1 | 1.9 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 5 mM cAMP, 180 mM ammonium acetate, 22.5% (w/v) PEG 3350, 90 mM TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |