3UKN
Structure of the C-linker/CNBHD of zELK channels in C 2 2 21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.980, 95.480, 241.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.558 - 2.200 |
| R-factor | 0.2146 |
| Rwork | 0.212 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Structure of the corresponding selenomethionine derivative crystal |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.092 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.560 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 | 0.434 |
| Number of reflections | 33782 | |
| <I/σ(I)> | 9.9 | 2.4 |
| Completeness [%] | 97.6 | 85.4 |
| Redundancy | 4.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.8 M non-detergent sulfobetaine (NDSB)-211, 180 mM ammonium acetate, 22.5% (w/v) PEG 3350, 90 mM TRIS , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
