3UKG
Crystal structure of Rap1/DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 140 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.595, 102.944, 116.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.930 - 2.950 |
| R-factor | 0.1978 |
| Rwork | 0.191 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ign |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.480 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.930 |
| High resolution limit [Å] | 2.950 |
| Rmerge | 0.138 |
| Number of reflections | 10759 |
| Completeness [%] | 90.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 100mM Tris-HCl pH 8.0, 20% (v/v) PEGmme-550, 100mM CaCl2, and 5% (v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
