3UKD
UMP/CMP KINASE FROM SLIME MOLD COMPLEXED WITH ADP, CMP, AND ALF3
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.800, 78.800, 100.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.900 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23200 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.900 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.346 |
| Total number of observations | 101015 * | |
| Number of reflections | 24834 | |
| Completeness [%] | 93.0 | 79 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.5 | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | UmpKdicty | 0.64 (mM) | |
| 2 | 1 | 1 | ADP | 0.8 (mM) | |
| 3 | 1 | 1 | CMP | 0.8 (mM) | or UMP |
| 4 | 1 | 1 | Tris-HCl | 100 (mM) | |
| 5 | 1 | 1 | 30 (mM) | ||
| 6 | 1 | 1 | DTE | 40 (mM) | |
| 7 | 1 | 1 | 0.8 (mM) | or BeCl2 | |
| 8 | 1 | 1 | 4 (mM) | ||
| 9 | 1 | 2 | PEG4000 |
