3UKB
(R)-cEt-BNA decamer structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.022, 44.897, 44.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.750 - 1.420 |
| R-factor | 0.14883 |
| Rwork | 0.146 |
| R-free | 0.20819 |
| Structure solution method | MOLREP (CCP4) |
| Starting model (for MR) | 3ey2 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.429 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.750 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.062 | 0.574 |
| Number of reflections | 9792 | |
| <I/σ(I)> | 36.6 | 1.7 |
| Completeness [%] | 98.9 | 94.5 |
| Redundancy | 7.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 20 mM sodium cacodylate, 40 mM sodium chloride, 6 mM spermine tetrahydrochloride, 5% v/v MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
