3UK7
Crystal Structure of Arabidopsis thaliana DJ-1D
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97954, 0.97955, 0.98736 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.778, 75.214, 141.673 |
| Unit cell angles | 90.00, 96.87, 90.00 |
Refinement procedure
| Resolution | 45.100 - 2.050 |
| R-factor | 0.17657 |
| Rwork | 0.174 |
| R-free | 0.22477 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.953 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 70208 | |
| Completeness [%] | 97.5 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.22M NaCl, 0.1M Bis-Tri, 21% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
