3UK0
RPD_1889 protein, an extracellular ligand-binding receptor from Rhodopseudomonas palustris.
Replaces: 3SNRExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.272, 71.578, 105.002 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.300 - 1.490 |
R-factor | 0.1391 |
Rwork | 0.136 |
R-free | 0.19440 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.648 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.300 | 50.000 | 1.520 |
High resolution limit [Å] | 1.490 | 4.040 | 1.490 |
Rmerge | 0.099 | 0.071 | 0.638 |
Number of reflections | 53560 | ||
<I/σ(I)> | 10.3 | 2.07 | |
Completeness [%] | 98.3 | 99.9 | 82.2 |
Redundancy | 13.1 | 14.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 289 | 0.2 M magnesium sulfate, 20% PEG-4000, 10% glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |