3UK0

RPD_1889 protein, an extracellular ligand-binding receptor from Rhodopseudomonas palustris.

Replaces:  3SNR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	43.272, 71.578, 105.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.300 - 1.490
R-factor	0.1391
Rwork	0.136
R-free	0.19440
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.648
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.300	50.000	1.520
High resolution limit [Å]	1.490	4.040	1.490
Rmerge	0.099	0.071	0.638
Number of reflections	53560
<I/σ(I)>	10.3		2.07
Completeness [%]	98.3	99.9	82.2
Redundancy	13.1	14.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	289	0.2 M magnesium sulfate, 20% PEG-4000, 10% glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 289K