3UJZ
Crystal structure of enterohemorrhagic E. coli StcE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.530, 186.000, 188.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.170 - 2.500 |
| R-factor | 0.2134 |
| Rwork | 0.211 |
| R-free | 0.25250 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.270 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 47011 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.2M MgCl2, 8% PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
