3UJT
Structure of the Fab fragment of Ab-52, an antibody that binds the O-antigen of Francisella tularensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2010-09-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.953, 131.491, 86.805 |
Unit cell angles | 90.00, 96.87, 90.00 |
Refinement procedure
Resolution | 14.842 - 2.100 |
R-factor | 0.1873 |
Rwork | 0.186 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1RJL heavy chain and PDB entry 3GI8 light chain |
RMSD bond length | 0.002 |
RMSD bond angle | 0.622 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX ((AutoMR)) |
Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.075 | 0.337 |
Number of reflections | 46183 | |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1 M tris, 24% w/v PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |