3UJQ
Galactose-specific lectin from Dolichos lablab in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 32 |
Unit cell lengths | 89.990, 89.990, 125.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.340 - 2.060 |
R-factor | 0.19895 |
Rwork | 0.197 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bjq |
RMSD bond length | 0.005 |
RMSD bond angle | 0.838 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 125.110 | 2.180 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.118 | 0.307 |
Number of reflections | 69408 | |
<I/σ(I)> | 8.8 | 4 |
Completeness [%] | 99.6 | 98.3 |
Redundancy | 4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 30% PEG 4K, 100mM Tris HCl, 200mM Mg-acetate , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |