Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 116.840, 116.840, 117.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.480 - 3.510 |
R-factor | 0.25894 |
Rwork | 0.257 |
R-free | 0.28918 |
Structure solution method | SIRAS |
Starting model (for MR) | Gin Homology Model of subunit A of 1ZR4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.988 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.500 |
Number of reflections | 6313 |
<I/σ(I)> | 8.59 |
Completeness [%] | 99.0 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 10% Ammonium Sulfate, 20% Ethylene Glycol, 10 mM MgSO4, 100 mM MES 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 10% Ammonium Sulfate, 20% Ethylene Glycol, 10 mM MgSO4, 100 mM MES 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |