3UJ2

CRYSTAL STRUCTURE OF AN ENOLASE FROM ANAEROSTIPES CACCAE (EFI TARGET EFI-502054) WITH BOUND MG AND SULFATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-15
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	282.350, 282.210, 119.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.029 - 2.000
R-factor	0.1728
Rwork	0.172
R-free	0.23090
Structure solution method	1e9i
Starting model (for MR)	1e9i
RMSD bond length	0.009
RMSD bond angle	1.045
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	202194
Completeness [%]	63.0	10
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	Protein (10 mM Hepes, pH 7.8, 150 mM NaCl, 10% glycerol, 5 mM DTT, 5 mM MgCl2; Reservoir (2M Ammonium Sulfate, 100 mM Citrate pH 5.5); Cryoprotection (2M Magnesium Sulfate, 100 mM Bis-Tris pH 5.3, 20% glycerol), VAPOR DIFFUSION, SITTING DROP, temperature 298K