3UIY
Crystal structure of SefD_dscA in H2O
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.480, 52.480, 218.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.450 - 3.100 |
| R-factor | 0.27027 |
| Rwork | 0.268 |
| R-free | 0.33151 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2axw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.811 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.450 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.072 | 0.443 |
| Number of reflections | 3659 | |
| <I/σ(I)> | 15.2 | 3.2 |
| Completeness [%] | 98.7 | 99.5 |
| Redundancy | 4.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 4 M ammonium acetate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
