3UIO

Complex between human RanGAP1-SUMO2, UBC9 and the IR1 domain from RanBP2 containing IR2 Motif II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	151.351, 151.351, 57.442
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.775 - 2.602
R-factor	0.2061
Rwork	0.204
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z5s
RMSD bond length	0.009
RMSD bond angle	1.184
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.690
High resolution limit [Å]	2.602	2.602
Rmerge	0.067	0.439
Number of reflections	23329
<I/σ(I)>	14.3	3.5
Completeness [%]	99.5	99
Redundancy	4.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	279	14% PEG4000, 100 mM sodium citrate pH 6.0, 200 mM ammonium acetate, vapor diffusion, hanging drop, temperature 279K