3UIA
Structural Determinants of Trimerization Specificity in HIV-1 gp41 Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97927 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.027, 76.316, 65.731 |
Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
Resolution | 63.500 - 2.000 |
R-factor | 0.21378 |
Rwork | 0.212 |
R-free | 0.25227 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u91 |
RMSD bond length | 0.026 |
RMSD bond angle | 1.886 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.500 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.431 |
Number of reflections | 25339 | |
<I/σ(I)> | 14.7 | 2.7 |
Completeness [%] | 99.2 | 99.5 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 34% MPD, 0.2M ammonium dihydrogen phosphate, 0.1M Tris, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |