3UI0
HBI (T72G) deoxy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-30 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.760, 44.430, 143.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.720 - 1.800 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4sdh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.720 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.276 |
Number of reflections | 26210 | |
<I/σ(I)> | 18.8 | 4.6 |
Completeness [%] | 94.4 | 94.2 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 8.5 | 298 | 1.5-2.5 M phosphate, pH 8.50, SMALL TUBES, temperature 298K |