3UHO
Crystal Structure of Glutamate Racemase from Campylobacter jejuni subsp. jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.308, 74.384, 50.144 |
| Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
| Resolution | 34.601 - 2.200 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uhf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.873 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_851)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 25698 | |
| <I/σ(I)> | 7.9 | 3.7 |
| Completeness [%] | 98.3 | 97.7 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2M Calcium Chloride, 0.1M Tris pH8.0, 16% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
