3UHA
Crystal Structure of Saccharopine Dehydrogenase from Saccharomyces cervisiae complexed with NAD.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-01-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.010, 104.450, 69.080 |
Unit cell angles | 90.00, 116.60, 90.00 |
Refinement procedure
Resolution | 32.640 - 2.300 |
R-factor | 0.21022 |
Rwork | 0.207 |
R-free | 0.26995 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 3UGK |
RMSD bond length | 0.019 |
RMSD bond angle | 1.885 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.640 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.122 | 0.632 |
Number of reflections | 35383 | |
<I/σ(I)> | 5.3 | 1.5 |
Completeness [%] | 98.1 | 96.9 |
Redundancy | 2.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M Bis-Tris Propane, 0.22M Malonate, 24% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |