3UGT

Crystal structure of the yeast mitochondrial threonyl-tRNA synthetase - orthorhombic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	200
Detector technology	CCD
Collection date	2010-08-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	154.083, 157.939, 237.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.113 - 3.600
R-factor	0.2391
Rwork	0.237
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.729
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Number of reflections	33252
<I/σ(I)>	10.7	1.73
Completeness [%]	97.5	83.8
Redundancy	6.4	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	285	0.2M magnesium formate, 20% PEG 3.350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 285K