3UGS
Crystal structure of a probable undecaprenyl diphosphate synthase (uppS) from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.643, 67.234, 118.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.457 |
R-factor | 0.20686 |
Rwork | 0.202 |
R-free | 0.25262 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dr2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.529 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.490 |
High resolution limit [Å] | 2.457 | 2.450 |
Rmerge | 0.068 | |
Number of reflections | 18362 | |
<I/σ(I)> | 28 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 279 | 45% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 279K |