3UGQ
Crystal structure of the apo form of the yeast mitochondrial threonyl-tRNA synthetase determined at 2.1 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-08-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.970, 67.970, 191.367 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.461 - 2.100 |
R-factor | 0.2082 |
Rwork | 0.205 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.882 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 26891 | |
<I/σ(I)> | 28.4 | 3.7 |
Completeness [%] | 98.6 | 87 |
Redundancy | 19.3 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 285 | 0.1M Hepes, pH 7.5, 2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 285K |