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3UGQ

Crystal structure of the apo form of the yeast mitochondrial threonyl-tRNA synthetase determined at 2.1 Angstrom resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]77
Detector technologyCCD
Collection date2010-08-10
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1.0000
Spacegroup nameP 43 21 2
Unit cell lengths67.970, 67.970, 191.367
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution33.461 - 2.100
R-factor0.2082
Rwork0.205
R-free0.24960
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.005
RMSD bond angle0.882
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.7_650))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.140
High resolution limit [Å]2.1002.100
Number of reflections26891
<I/σ(I)>28.43.7
Completeness [%]98.687
Redundancy19.36
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.52850.1M Hepes, pH 7.5, 2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 285K

217705

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