3UGP
Crystal structure of RNA-polymerase sigma subunit domain 2 complexed with -10 promoter element ssDNA oligo (TATAAT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-08-09 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 4 2 2 |
Unit cell lengths | 86.001, 86.001, 105.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.220 - 2.697 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ku2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.425 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.5_2) |
Refinement software | PHENIX ((PHENIX.REFINE: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.697 | 2.697 |
Rmerge | 0.105 | 0.520 |
Number of reflections | 11450 | |
<I/σ(I)> | 31.63 | 6.77 |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.8 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 5% PEG8000, 20% PEG300, 10% glycerol, 0.15% mellitic acid, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |