3UGK
Crystal Structure of C205S mutant and Saccharopine Dehydrogenase from Saccharomyces cerevisiae.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-10-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.950, 75.230, 75.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.700 - 2.010 |
R-factor | 0.20126 |
Rwork | 0.197 |
R-free | 0.27275 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.741 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.700 | 2.120 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.052 | 0.295 |
Number of reflections | 24396 | |
<I/σ(I)> | 15.8 | 3 |
Completeness [%] | 97.1 | 81.8 |
Redundancy | 4 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG-MME 2000, Tris buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |