3UGB

UbcH5c~Ubiquitin Conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	113
Detector technology	CCD
Collection date	2011-08-09
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	42.027, 52.510, 53.114
Unit cell angles	90.00, 101.07, 90.00

Refinement procedure

Resolution	29.618 - 2.350
R-factor	0.213
Rwork	0.211
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3TGD AND 1UBQ
RMSD bond length	0.014
RMSD bond angle	1.071
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX (1.7.2_869)
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.510	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.048	0.204
Number of reflections	9319
<I/σ(I)>	19.2	5
Completeness [%]	97.1	78.2
Redundancy	4.89	3.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2M tri-potassium citrate, 20% PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K