3UG9
Crystal Structure of the Closed State of Channelrhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.471, 139.114, 90.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.569 - 2.300 |
| R-factor | 0.2088 |
| Rwork | 0.204 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.800 | 2.440 | |
| High resolution limit [Å] | 2.300 | 6.830 | 2.300 |
| Rmerge | 0.080 | 0.024 | 0.936 |
| Rmeas | 0.026 | 1.017 | |
| Number of reflections | 18985 | 709 | 2581 |
| <I/σ(I)> | 16.5 | 55.27 | 1.94 |
| Completeness [%] | 97.9 | 97.7 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipid cubic phase | 6 | 293 | 30% PEG 500 DME, 100mM Na Citrate (pH6.0), 100mM MgCl2, 100mM NaCl, 100mM (NH4)2SO4, lipid cubic phase, temperature 293K |
