3UG9

Crystal Structure of the Closed State of Channelrhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	59.471, 139.114, 90.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.569 - 2.300
R-factor	0.2088
Rwork	0.204
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.800		2.440
High resolution limit [Å]	2.300	6.830	2.300
Rmerge	0.080	0.024	0.936
Rmeas		0.026	1.017
Number of reflections	18985	709	2581
<I/σ(I)>	16.5	55.27	1.94
Completeness [%]	97.9	97.7	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	lipid cubic phase	6	293	30% PEG 500 DME, 100mM Na Citrate (pH6.0), 100mM MgCl2, 100mM NaCl, 100mM (NH4)2SO4, lipid cubic phase, temperature 293K