3UG4
Crystal structure of alpha-L-arabinofuranosidase from Thermotoga maritima arabinose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.483, 160.639, 154.841 |
Unit cell angles | 90.00, 92.44, 90.00 |
Refinement procedure
Resolution | 29.990 - 2.150 |
R-factor | 0.16924 |
Rwork | 0.167 |
R-free | 0.20666 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pz3 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.837 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 266042 | |
<I/σ(I)> | 20.8 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M sodium citrate, 0.1M Tris-HCl, 0.2M NaCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |