3UFE
Structure of transcriptional antiterminator (BGLG-family) at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.360, 65.810, 38.290 |
| Unit cell angles | 90.00, 109.62, 90.00 |
Refinement procedure
| Resolution | 36.070 - 1.500 |
| R-factor | 0.15892 |
| Rwork | 0.157 |
| R-free | 0.19961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gwh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.472 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.070 | 1.600 |
| High resolution limit [Å] | 1.496 | 1.500 |
| Rmerge | 0.046 | 0.341 |
| Number of reflections | 27940 | |
| <I/σ(I)> | 18.6 | 3.16 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 5.41 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | 2.4 M ammonium sulfate, 0.1 M citric acid, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
