3UFC
Characterization of a Cas6-related gene from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 79.805, 79.805, 117.362 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.030 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.257 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.030 | 2.090 |
Number of reflections | 26932 | |
Completeness [%] | 94.2 | 97.1 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 30% (w/v) polyethylene glycol 400, 0.1M HEPES-Na, 0.1M Magnesium chloride hexahydrate , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |