3UFB

Crystal structure of a modification subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Detector technology	CCD
Collection date	2008-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	78.899, 78.899, 165.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.999 - 1.800
R-factor	0.1844
Rwork	0.181
R-free	0.21540
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.006
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	48972
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20 % (w/v) polyethylene glycol 8,000, 0.1 M at Hepes pH 7.5 , VAPOR DIFFUSION, HANGING DROP, temperature 291K
2	VAPOR DIFFUSION, HANGING DROP	7.5	291	24 % (w/v) polyethyleneglycol 3350, 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid at pH 7.5 and 10 mM -mercaptoethanol , VAPOR DIFFUSION, HANGING DROP, temperature 291K