3UF7
Co-crystal structure of Escherichia coli uracil-DNA glycosylase and a C-terminal fragement of the single-stranded DNA-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-05-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | I 4 |
| Unit cell lengths | 118.204, 118.204, 47.098 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.200 |
| R-factor | 0.1401 |
| Rwork | 0.139 |
| R-free | 0.15610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lqg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.749 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 3.260 | 1.200 |
| Rmerge | 0.048 | 0.045 | 0.417 |
| Number of reflections | 99762 | ||
| <I/σ(I)> | 17.8 | ||
| Completeness [%] | 98.2 | 92 | 83.9 |
| Redundancy | 4.8 | 8.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.4-1.8M NH4SO4, 0.01M MgCl2 & 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
