3UEV
Bovine beta-lactoglobulin complex with myristic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2009-10-19 |
Detector | AGILENT ATLAS CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 53.158, 53.158, 111.131 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.180 - 1.900 |
R-factor | 0.22802 |
Rwork | 0.223 |
R-free | 0.27491 |
Structure solution method | MOLREP |
Starting model (for MR) | 1bsy |
RMSD bond length | 0.016 |
RMSD bond angle | 1.659 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.180 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.309 |
Number of reflections | 14911 | |
<I/σ(I)> | 13.5 | 3.3 |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.34 M trisodium citrate, 0.1 M Tris-HCl buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |