3UEO
Crystal structure of TopBP1 BRCT4/5 domains in complex with a phospho-peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2011-02-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 |
Unit cell lengths | 58.810, 59.100, 78.310 |
Unit cell angles | 102.05, 98.04, 114.34 |
Refinement procedure
Resolution | 34.566 - 2.600 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.132 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.600 | 2.700 | |
High resolution limit [Å] | 2.600 | 10.000 | 2.600 |
Rmerge | 0.059 | 0.018 | 0.402 |
Number of reflections | 26653 | 460 | 2880 |
<I/σ(I)> | 11.89 | 38.6 | 2.23 |
Completeness [%] | 95.2 | 94.1 | 95.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 10000, Ammonium acetate, bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |