3UE6

The dark structure of the blue-light photoreceptor Aureochrome1 LOV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 43
Unit cell lengths	73.987, 73.987, 177.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.088 - 2.750
R-factor	0.1956
Rwork	0.192
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.044	0.490
Number of reflections	24720
<I/σ(I)>	34.1	2
Completeness [%]	99.8	100
Redundancy	7.3	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	100 mM phosphate-citrate buffer (pH 4.2), 20% (w/v) PEG 8000, and 200 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K