3UE5

ECP-cleaved Actin in complex with Spir domain D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	84.837, 53.551, 100.578
Unit cell angles	90.00, 92.65, 90.00

Refinement procedure

Resolution	50.240 - 2.760
R-factor	0.1995
Rwork	0.197
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (1.3.2)
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	2.900
High resolution limit [Å]	2.760	6.030	2.800
Rmerge	0.105	0.075	0.237
Number of reflections	10542
<I/σ(I)>	8.8
Completeness [%]	89.6	97.4	55.4
Redundancy	2.7	2.7	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	Tris pH 8.5, magnesium chloride, PEG-8000, vapor diffusion, hanging drop, temperature 298K