3UE2
Crystal structure of a RNA binding domain of poly-U binding splicing factor 60KDa (PUF60) from Homo sapiens at 1.23 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537,0.9796,0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.640, 49.995, 55.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.553 - 1.230 |
| R-factor | 0.1355 |
| Rwork | 0.134 |
| R-free | 0.16010 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.506 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.553 | 29.553 | 1.260 |
| High resolution limit [Å] | 1.230 | 5.500 | 1.230 |
| Rmerge | 0.051 | 0.612 | |
| Total number of observations | 1173 | 7515 | |
| Number of reflections | 30048 | ||
| <I/σ(I)> | 8.3 | 12.1 | 1.3 |
| Completeness [%] | 99.8 | 96.5 | 100 |
| Redundancy | 3.4 | 3.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 2.4 M ammonium sulfate, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
