3UE2

Crystal structure of a RNA binding domain of poly-U binding splicing factor 60KDa (PUF60) from Homo sapiens at 1.23 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537,0.9796,0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	36.640, 49.995, 55.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.553 - 1.230
R-factor	0.1355
Rwork	0.134
R-free	0.16010
Structure solution method	MAD
RMSD bond length	0.024
RMSD bond angle	1.506
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.553	29.553	1.260
High resolution limit [Å]	1.230	5.500	1.230
Rmerge		0.051	0.612
Total number of observations		1173	7515
Number of reflections	30048
<I/σ(I)>	8.3	12.1	1.3
Completeness [%]	99.8	96.5	100
Redundancy	3.4	3.1	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	2.4 M ammonium sulfate, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K