3UDT
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from A. thaliana in complex with ADP and IP5.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-08-14 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 |
Unit cell lengths | 59.362, 59.913, 83.146 |
Unit cell angles | 90.02, 97.04, 116.81 |
Refinement procedure
Resolution | 44.629 - 3.100 |
R-factor | 0.2134 |
Rwork | 0.210 |
R-free | 0.27620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.287 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.6.3_473) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 32677 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.09M MES, 18% PEG4000, 0.54M NaCl, 0.01M Trimethylamine HCl, Protein: 5mg/mL, Temp: 293K, pH 6.5, vapor diffusion |