3UD7
Crystal Structure Analysis of FGF1-Disaccharide(NI21) complexes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 115.149, 47.077, 98.874 |
Unit cell angles | 90.00, 125.32, 90.00 |
Refinement procedure
Resolution | 80.670 - 2.800 |
R-factor | 0.19583 |
Rwork | 0.188 |
R-free | 0.26726 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rg8 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.621 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.670 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.084 | 0.438 |
Number of reflections | 10803 | |
<I/σ(I)> | 7.4 | 2 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2M (NH4)HPO4, 0.1M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |