3UD2
Crystal structure of Selenomethionine ZU5A-ZU5B protein domains of human erythrocyte ankyrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97626 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 279.480, 40.873, 95.249 |
Unit cell angles | 90.00, 92.01, 90.00 |
Refinement procedure
Resolution | 24.900 - 2.210 |
R-factor | 0.22258 |
Rwork | 0.221 |
R-free | 0.25878 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.346 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.042 | |
Number of reflections | 55529 | |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283.15 | 25% ethanol, 0.1 M Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 283.15K |