3UD2

Crystal structure of Selenomethionine ZU5A-ZU5B protein domains of human erythrocyte ankyrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97626
Spacegroup name	C 1 2 1
Unit cell lengths	279.480, 40.873, 95.249
Unit cell angles	90.00, 92.01, 90.00

Refinement procedure

Resolution	24.900 - 2.210
R-factor	0.22258
Rwork	0.221
R-free	0.25878
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.346
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.700	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.042
Number of reflections	55529
<I/σ(I)>	14.2
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	283.15	25% ethanol, 0.1 M Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 283.15K