3UCQ
Crystal structure of amylosucrase from Deinococcus geothermalis
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.316, 110.203, 115.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.220 - 1.970 |
| R-factor | 0.14676 |
| Rwork | 0.145 |
| R-free | 0.18751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g5a |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.842 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.520 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 44959 | |
| Completeness [%] | 99.3 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 1.5 M sodium acetate, 0.1 M sodium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |
