3UCI
Crystal structure of Rhodostomin ARLDDL mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.221, 33.437, 73.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.350 |
| R-factor | 0.14729 |
| Rwork | 0.145 |
| R-free | 0.18924 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j2l |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.284 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.055 | 0.559 |
| Number of reflections | 14629 | |
| <I/σ(I)> | 34.15 | 4.76 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, 0.8M sodium citrate tribasic dihydrate, 0.24M lithium sulfide, 0.5% glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
